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KMID : 0376419960200010097
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Chonbuk University Medical Journal
1996 Volume.20 No. 1 p.97 ~ p.102
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Antitumor effect of Korean Ginseng: Cytotoxicity and Tumor growth delay(1)
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Abstract
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Purpose:
@EN To investigate the effect of aqueous extract of Korean Ginseng(K.G) on the survival of tumor cells in vitro and on the growth of tumors in vivo.
@ES Materials and Methods:
@EN Dried K.G. roots were made into powder, extracted with distilled water, filtered and diluted from a maximum concentration of 100mg/㎖ in sequence. The cytotoxicity of K.G. in vitro was evaluated from its ability to reduce the clonogenicity of
SCK
tumor cells. For the tumor growth delay study, about 2×10E5 of SCK tumor cells were subcutaneously inoculated in the legs of A/J mice. The first experimental group of mice were injected intraperitoneally(i.p.) with 0.2㎖ of 500mg/kg of K.G. from
the
first day after tumor inoculation for 10 days. The second experimental group of mice were injected i.p. with 0.2㎖ of 500mg/kg of K.G. twice a day for 5 days beginning from the 7th day after tumor inoculation.
@ES Results:
@EN (1) Cytotoxity in vitro; survival fraction, as judged from the curve, at K.G. concentration of 1, 5, 10, 25, 50 and 100mg/㎖ were 1.0±0.02, 0.58±0.07, 0.84±0.03, 0.25±0.02, 0.004 and 0.002 respectively. In the concentration-cell survival
fraction relationship, the range of ginseng concentration at 10% of cell survival fraction was 25~50mg/㎖. (2) Tumor growth delay in vivo: 1) the time required for the mean tumor volume to grow to 1,000㎣was 11 days in the control group and 12
days
in
the experimental group(p>0.05). 2) the time required for tumor volume to increase 4 times was 11 days in both groups.
@ES Conclusion:
@EN Aqueous extract of dried K.G. roots showed a marked cytotoxicity on the SCK mammary cells in vitro and the range of ginseng concentration at 10% cell survival fraction was 25~50mg/㎖. When K.G. injection was started soon after tumor
inoculation,
tumor growth was delayed than that of control group, but it was not significant was started after the tumors were firmly established, difference of tumor growth delay was not appeared in both group.
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KEYWORD
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